cat pratical case

The cement pond system is used to breed grouper, and a new outbreak of nerve necrosis virus has occurred, resulting in about 100+deaths per day. Samples of diseased fish were taken for testing, and the results showed positive for nerve necrosis virus, negative for rainbow virus, and no bacterial infection.

At a bullfrog farm in Chenghai, two adjacent frog ponds with similar sizes were selected, one as the control group and the other as the experimental group. Two frog ponds with the same number of frogs (both 10W) were used, and the experimental group and the control group were kept under the same breeding conditions. The experimental group used frog tilapia (take 2 packets of frog tilapia and 600ml of vegetable oil, mix thoroughly, and then mix the mixed frog tilapia with 20kg of feed evenly for feeding frogs). Feed once a day for 3 consecutive days; After an interval of one month, feed again for three consecutive days, with one meal per day, while keeping other routine health medications consistent with the control group.

The feed mandarin fish in the farm died continuously, with 20-60 deaths per day. Disease samples were taken multiple times for testing, and the test results showed strong positive for splenic and renal necrosis virus nucleic acid (CT value of about 10) and negative for frog rainbow virus nucleic acid, confirming that the feed mandarin fish were infected with splenic and renal necrosis virus disease.